Effect of eugenolon white blood cells and corticosterone in sub-acute restraint stress-induced wistar albino rats

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Original Article

Author Details : M Pandian, Padmaja R Desai*

Volume : 6, Issue : 4, Year : 2019

Article Page : 433-437

https://doi.org/10.18231/j.ijcap.2019.094

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Abstract

Introduction: The effect of eugenol on white blood cells (WBCs ) and corticosteroneis known. However,
pharmacological properties and activities of eugenol such as anti-inflammatory, antioxidant, anesthetic,
muscle relaxant, improved motor coordination, and increased red blood cells were reported in stress -
induced rats.
Objective: To evaluate the effect of eugenol on WBCs in sub -acute restraint stress-induced Wistar albino
rats.
Materials and Methods: Female Wistar albino rats (n = 30) weighing (150–220 g) were divided into five
groups with six animals in each group, Group I: normal control; II : vehicle polyglycerol treated (PG); III:
eugenol treated alone - TA (150 mg/kg/i.p for 15 days); IV : stress -induced (SA-stress alone), and V: stress
followed by treated (T/S) with. Blood samples were collected at the end ofthestudyand total leucocyte count
(TLC), differentiation leucocyte count (DLC), platelet count (PC) and concentration of corticosterone were
estimated.
Results: The TA group showed significant (p<0> group. The platelet count was significantly less in the SA group as compared to T/ S group (p<0> effect of eugenol on DLC did not show a significant difference with respect to neutrophil and lymphocytes
count in T/S groups, when compared with other groups, whereas, no significant differences were observed
between basophils, monocytes, and eosinophils of T/S when compared with SA group. No significant
differences were observed in corticosterone levels in all groups.
Conclusion: Acute restraint stress induced distribution of WBC was not significantly attenuated by eugenol
and no changes were observed in corticosterone level. However, eugenol increases platelet aggregation.

Keywords: Stress, Eugenol, Total leukocyte count, Differentiation leukocyte count, Platelets, Corticosterone.

How to cite : Pandian M, Desai P R, Effect of eugenolon white blood cells and corticosterone in sub-acute restraint stress-induced wistar albino rats. Indian J Clin Anat Physiol 2019;6(4):433-437

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